The NanoVision Centre at Queen Mary University of London (Mile End campus)

Jon is the TEM Biological sciences representative on NanoVision's management group.

In this capacity, the following webpages may provide news and support for the NanoVision Biological sciences TEM user base, both for internal and external users.

Any query re: Biological TEM use is welcomed here, ultimately fielded by the centre's Experimental Officer: Dr. Nadezhda (Nadja) Tarakina, x8468.
n.tarakina 'at' qmul.ac.uk or by Russell Bailey, technical support, x8468. r.j.bailey ‘at’ qmul.ac.uk

*News/Diary* for Jon (with Kristina, Karim or Liang) - please follow my NanoVision blog

[Jeol 1230 TEM Bookings page (℅ Harold Toms) -
http://n52-014.sbcs.qmul.ac.uk/]

TEM Equipment available for structural studies:
Instrumentation within Queen Mary's NanoVision centre is centred around two instruments for primary data collection (room temperature, +/- negative stain):

Jeol 2010, with Gatan Orius SC1000 digital camera - Jan. 2011.
Jeol 1230, attached side-entry (retractable) CCD camera is a Morada, 2672 x 2672 pixel (2k); each element 9 micron x 9 micron (physical size).

For the Jeol 1230 TEM / Morada CCD system:
30,000 x = 16 Å / pixel
50,000 x = 9.582 Å / pixel
60,000 x = 7.969 Å / pixel
80,000 x = 5.962 Å / pixel

C.s. = 3.0 mm

[ Further equipment available within the centre: see
main webpages (beyond this page's remit). e.g. FEI Inspect F (S/TEM), FEI Quanta 3D Environmental SEM (cryo-capable) ]

- PELCO easiGlow - Glow discharge unit (plasma cleaner)
Analytical lab, Joseph Priestley building
See Jon/Kristina for training/first use - manuals/safety (.pdf easy guide available on request)

- Intel/Opteron G34 quad-core, quad-socket image-processing servers (160 node; 64/128 GB RAM; 24 TB NAS)

- Nikon LS9000 CoolScan densitometer (for film option):
The lab has a Nikon LS9000 scanner, 6.35 micrometre step-size to scan at 16 bit greyscale (6 x 7.5 cm; holder specially modified (machined) to enable 6 x 9 cm film to be scanned); typical total cycle scan-time per single micrograph is ~ 5 min, producing a scan of ~ 200 Mb per micrograph. Tests specific to this machine have concluded a 6 Angstrom 3D structure is achievable, assuming scanner is rate limiting step. The scanner connects by a standard firewire interface coupled to a SFF PC with Nikon Scan 4.1 software. Users are encouraged to save data on their own external storage solution (e.g. USB 3.0, Samsung 4 TB).

Recent TEM-based publications (Nield J) :

Albanese P., Nield J., Tabares J.A.M., Chiodoni A., Manfredi M., Gosetti F., Marengo E., Saracco G., Barber J. & Pagliano C.
Isolation of novel PSII-LHCII megacomplexes from pea plants characterized by a combination of proteomics and electron microscopy.
(2015) Photosynthesis Research. Accepted. 31.12.2015.

Pagliano C., Nield J., Marsano F., Pape T., Barera S., Saracco G. & Barber J.
Proteomic characterization and three-dimensional electron microscopy study of PSII-LHCII supercomplexes from higher plants.
(2014) Biochim Biophys Acta. 1837(9):1454-1462.
Epub. Nov 16. 2013 doi:pii: S0005-2728(13)00189-8. 10.1016/j.bbabio.2013.11.004.

Gutiérrez L., Zubow K., Nield J., Gambis A., Mollereau B., Lázaro F.J., Missirlis F.
Biophysical and genetic analysis of iron partitioning and ferritin function in Drosophila melanogaster.
(2013) Metallomics. 5(8):997-1005. doi: 10.1039/c3mt00118k.

Boehm M., Yu J., Krynicka V., Barker M., Tichy M., Komenda J., Nixon P.J., Nield J.
Subunit localisation of a hetero-oligomeric thylakoid FtsH complex involved in photosystem II repair.
(2012) Plant Cell. 24(9):3669-3683. doi: 10.1105/tpc.112.100891.

Nield, J., Moriya, M., Ochiai, M.
Calmodulin binding-heat shock proteins form a ring structure in the rat testis
(2010)
Molecular Reproduction and Development, 77(9):738. doi: 10.1002/mrd.21231.

Busch, A., Nield, J., Hippler, M.
The composition and structure of photosystem I-associated antenna from Cyanidioschyzon merolae
(2010)
Plant Journal, 62(5):886-97. doi: 10.1111/j.1365-313X.2010.04202.x. Epub 2010 Mar 10.

Ozawa, S.-I., Nield, J., Terao, A., Stauber, E.J., Hippler, M., Koike, H., Rochaix, J.-D., Takahashi, Y.
Biochemical and structural studies of the large ycf4-photosystem i assembly complex of the green alga Chlamydomonas reinhardtii
(2009)
Plant Cell, 21(8):2424-42. doi: 10.1105/tpc.108.063313. Epub 2009 Aug 21.