Principal Research Fellow (Faculty, Senior Lecturer in Structural Biology) Jon Nield, Ph.D. j.nield 'at' qmul.ac.uk Principal Investigator; JST/CREST, Special Advisor (2014-2015) Academic Visitor, Imperial College (2015-2016)
Research Fellow Karim Maghlaoui, Ph.D. (& Support Worker) k.maghlaoui ‘at’ qmul.ac.uk
Equipment used in researching photosynthetic complexes: - for structural studies Instrumentation (JEOL 1230 / JEOL 2010 TEMs) within Queen Mary's NanoVision centre is used to record image data via CCD (2k x 2k; 16 bit TIFF) prior to single particle image processing across a wide range of local and collaborative projects.
Pre-2007, data for single particle image averaging projects were gained through the use of FEI (Philips) CM100 (100 keV), Tecnai T12 (120 keV) or CM200 (200 keV) FEG (Field Emission Gun; liquid Nitrogen-cooled) transmission electron microscopes.
A Linux cluster for the group has been recently upgraded and reconfigured (160-CPU, 336 Gb RAM, GPGPUs) for local image-processing. Our School, SBCS, is awaiting installation of a new 200 keV cryo-TEM facility during 2018 (PI: R.Pickersgill, BBSRC-funded). Jon is a co-PI member of the LonCEMcommunity. - for investigating biochemical function Our dark lab in The Joseph Priestley building has access to a wide range of equipment: Absorption spectroscopy, SDS-PAGE, Western blotting, HPLC (size exclusion and reverse-phase), FPLC, gas chromatography, gel densitometry, oxygen electrodes, fluorescence induction and relaxation, crystallisation, plant/ bacterial growth greenhouses/culturing, autoclaves, several centrifuges (+ultras) are all available, either within the lab, down the corridor, or within 5 min walk. Legacy equipment: Nikon LS9000 CoolScan densitometer If required, a Nikon scanner is able to provide for 6.35 micron step-size scans of electron micrographs, at 16 bit greyscale (6 x 7.5 cm; holder manually machined to enable 6 x 9 cm films to be scanned). A typical total cycle scan-time per single micrograph is ~ 5 min. After testing, a 4 Angstrom structure is possible, but 6 Angstroms clearly achievable.
Lab Alumni: Dr Liang Wang Dr Ray Burton-Smith Dr Kristina Zubow Mr Matt Cullen Mme Clemence Granier Mr Boaz Odier Dr (Ms) Jawon Song Dr William Lee Ms Vin Luang Mr Wilfred Wu Dr Dorothy Cheng Ms Sali Liu Ms Candy Xu Ms Connie Zhang Dr Yuan-Tsan Tseng Dr Ben King Dr Sven Nottebaum Ms Lu Pan Mr Laurence Darby Dr Jonathan Ruprecht